Continuous Culture Technique used for Strain
Improvement
Culture technique provides the growth features of microbial agent, the substrate conversion by the time and how the substrate utilizes. This technique explains how the Batch culture, Fed-batch culture and Continuous culture influence the culture growth. During this system we track the biomass and nutrient concentration.
It is the technique used for strain improvement and is an “open-culture system” for the cultivation of microbes or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. In this technique nutrients are added to the fermenter to increase the volume of medium in vessels continuously and products are removed at same rate. If the substrate is added more than we need then we lose the flow rate regulator and if is added at different intervals then the process become halt.
тs=тp
Substrate added Product obtained
тs : Time of substrate added
тp : Time of product removed
It is the most common type of continuous culture method. Chemostats allow for steady state concentration of growth limiting substrates to be maintained at a fixed level in the culture fluid, which results in highly reproducible ‘steady state’ growth conditions in which changes in cell density, physiological state, and medium composition of the culture are no longer detectable. Growth under the nutrient limitation results in submaximal growth rates, which can usually emulate the state of most natural environments far better than what can be achieved with batch cultures.
In continuous culture, nearly all environmental parameters including;
↺↺↺↺↺Figure 1 Continuous Culture (Chemostat)
Culture Technique
Culture technique provides the growth features of microbial agent, the substrate conversion by the time and how the substrate utilizes. This technique explains how the Batch culture, Fed-batch culture and Continuous culture influence the culture growth. During this system we track the biomass and nutrient concentration.
Continuous Culture Technique:
It is the technique used for strain improvement and is an “open-culture system” for the cultivation of microbes or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. In this technique nutrients are added to the fermenter to increase the volume of medium in vessels continuously and products are removed at same rate. If the substrate is added more than we need then we lose the flow rate regulator and if is added at different intervals then the process become halt.
Time required:
тs=тp
Substrate added Product obtainedтs : Time of substrate added
тp : Time of product removed
Chemostats:
It is the most common type of continuous culture method. Chemostats allow for steady state concentration of growth limiting substrates to be maintained at a fixed level in the culture fluid, which results in highly reproducible ‘steady state’ growth conditions in which changes in cell density, physiological state, and medium composition of the culture are no longer detectable. Growth under the nutrient limitation results in submaximal growth rates, which can usually emulate the state of most natural environments far better than what can be achieved with batch cultures.
Environmental parameters:
In continuous culture, nearly all environmental parameters including;
1) PH
2) Oxygen tension
3) Population density
4) Concentration of excretion products
can easily be controlled
↺↺↺↺↺Figure 1 Continuous Culture (Chemostat)
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